Laboratory of Nanobiotechnologies

Microplate reader CLARIOStar
Category:
Spectrophotometry and Optical devices
Applications:
in vitro
Description

The CLARIOstar is a multimode microplate reader with Advanced LVF Monochromators, highly sensitive filters, and an ultra-fast spectrometer. It is a modular microplate reader, having up to eight different detection modes, including: Fluorescence Intensity, FRET, Fluorescence Polarization, Luminescence, BRET, UV/Vis Absorbance, Time-Resolved Fluorescence, TR-FRET, and AlphaScreen® /AphaLISA®.

Besides the LVF Monochromators, the CLARIOstar can measure fluorescence and luminescence assays, including FRET and BRET, with fixed filters. Additionally, filters can be combined with the monochromators. Excite a fluorophore with a narrow bandwidth, high transmission filter and measure the emission with a broad bandwidth on the monochromator, or perform an emission spectral scan while exciting with a filter. Having filters that can be combined with a monochromator guarantees there is no compromise in performance in any fluorescence or luminescence assay. Moreover, all of these options are available to measure assays from both the top and bottom of the microplate.

In addition to filters and monochromators, the CLARIOstar incorporates an ultra-fast spectrometer for UV / Vis absorbance detection. This technology, pioneered several years ago by BMG LABTECH, allows users to spectrally scan an absorbance assay in less than 1 second per well. Users obtain an entire spectrum from 220 to 1000 nm at a 1 nm resolution; that is 780 distinct wavelengths in the same time other instruments measure only one or two. Full spectrum absorbance allows for assays not possible on other instruments.

Microplate reader CLARIOStar
Category:
Spectrophotometry and Optical devices
Applications:
in vitro
Description

The CLARIOstar is a multimode microplate reader with Advanced LVF Monochromators, highly sensitive filters, and an ultra-fast spectrometer. It is a modular microplate reader, having up to eight different detection modes, including: Fluorescence Intensity, FRET, Fluorescence Polarization, Luminescence, BRET, UV/Vis Absorbance, Time-Resolved Fluorescence, TR-FRET, and AlphaScreen® /AphaLISA®.

Besides the LVF Monochromators, the CLARIOstar can measure fluorescence and luminescence assays, including FRET and BRET, with fixed filters. Additionally, filters can be combined with the monochromators. Excite a fluorophore with a narrow bandwidth, high transmission filter and measure the emission with a broad bandwidth on the monochromator, or perform an emission spectral scan while exciting with a filter. Having filters that can be combined with a monochromator guarantees there is no compromise in performance in any fluorescence or luminescence assay. Moreover, all of these options are available to measure assays from both the top and bottom of the microplate.

In addition to filters and monochromators, the CLARIOstar incorporates an ultra-fast spectrometer for UV / Vis absorbance detection. This technology, pioneered several years ago by BMG LABTECH, allows users to spectrally scan an absorbance assay in less than 1 second per well. Users obtain an entire spectrum from 220 to 1000 nm at a 1 nm resolution; that is 780 distinct wavelengths in the same time other instruments measure only one or two. Full spectrum absorbance allows for assays not possible on other instruments.